About: Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase.

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About: Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. Goto Sponge NotDistinct Permalink

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article published in 2016

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rdf:type	Item
description	2016年學術文章 (zh) наукова стаття, опублікована у 2016 (uk) article published in 2016 (en)
publication date	wds:Q105109905-F0738742-CB48-4695-967B-0CD4ED813B21
publication date	2016-12-05 00:00:00Z (xsd:dateTime)
language of work or name	wds:Q105109905-98bcda45-40af-0172-9ed6-1a5d104a8d2e
language of work or name	English
cites work	wds:Q105109905-13213721-0159-4E6B-B72F-15A8F4E8B6A3 wds:Q105109905-32DD7037-8F80-4275-9DD5-E32353497278 wds:Q105109905-52BF77E1-1721-428A-9609-182052AC1573 wds:Q105109905-694EC9AF-6A04-4AE4-AE03-CCA27659D43A wds:Q105109905-7B8EBB3F-CD90-47F8-A830-0981864E4A9F wds:Q105109905-807FEA21-38D4-4D98-AED4-4DCC68E0D781 wds:Q105109905-82849278-11B5-45F6-877B-6F142C18AD6A wds:Q105109905-86DF3D7B-32C1-48E9-BEEF-00799D3D4250 wds:Q105109905-8A691A25-3185-4259-A652-73FB8B5FC531 wds:Q105109905-8CC1E49A-C0BE-47FD-BFFD-2B3DD3A49B54 wds:Q105109905-8EACA15F-D26E-4EAC-959A-9EF8A3AF1A97 wds:Q105109905-A16DFEF5-9BE7-46B7-9240-04C471A4ED24 wds:Q105109905-B4C3A4F5-9170-4403-9044-8DE6BAA1B0A4 wds:Q105109905-B94D8855-EC7C-415F-9A3A-FDAF197650DF wds:Q105109905-C4914F34-7913-44F4-857F-D7B99529A744 wds:Q105109905-E0E1BF97-BB30-4E67-992B-BE8AA0FF89E2 wds:Q105109905-EA31D343-ED65-4099-B1B2-27B26033463A wds:Q105109905-FB62A165-9A19-490F-99C8-621F9E3A9D2D wds:Q105109905-FB8200B0-5A1F-4F1D-B5AF-5866BA592D67
cites work	In VitroDeletional Mutagenesis for Bacterial Production of the 20,000-Dalton Form of Human Pituitary Growth Hormone A gas-liquid solid phase peptide and protein sequenator The relationship between base composition and codon usage in bacterial genes and its use for the simple and reliable identification of protein-coding sequences Cosmid shuttle vectors for cloning and analysis of Streptomyces DNA. Purification of isopenicillin N synthetase Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host Translation of a synthetic two-cistron mRNA in Escherichia coli Cloning genes for the biosynthesis of a macrolide antibiotic Isolation, sequence determination and expression in Escherichia coli of the isopenicillin N synthetase gene from Cephalosporium acremonium Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium Cloning and expression of the isopenicillin N synthetase gene from Penicillium chrysogenum Cloning and expression in Escherichia coli of isopenicillin N synthetase genes from Streptomyces lipmanii and Aspergillus nidulans A pure enzyme catalyzing penicillin biosynthesis Analysis of the role of cysteine residues in isopenicillin N synthetase activity by site-directed mutagenesis Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from Streptomyces clavuligerus Cloning and characterization of the isopenicillin N synthetase gene mediating the formation of the beta-lactam ring in Aspergillus nidulans. Deacetoxycephalosporin C synthetase and deacetoxycephalosporin C hydroxylase are two separate enzymes in Streptomyces clavuligerus Purification and characterization of a 2-oxoglutarate-linked ATP-independent deacetoxycephalosporin C synthase of Streptomyces lactamdurans Partial purification and catalytic properties of a bifunctional enzyme in the biosynthetic pathway of beta-lactams in Cephalosporium acremonium
access restriction status	wds:Q105109905-648CB522-C9C8-410A-B15E-72087AC9ECF2
access restriction status	open access
author name string	wds:Q105109905-27F56546-28C9-48AF-A74B-FB04EAA8FDD1 wds:Q105109905-7C4CC771-318B-4679-A703-E56D3C90DB98 wds:Q105109905-A20C374B-7FCF-45B9-BFB7-CC1A8A86B675 wds:Q105109905-A25CF9C1-3BF6-4748-9CD5-188DA9E034E0 wds:Q105109905-E445F0C9-6FBB-45AA-AC11-C77A6FD1112E
author name string	J R Miller T D Ingolia M B Tobin S Kovacevic B J Weigel
rdfs:label	Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. (en)
skos:prefLabel	Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. (en)
name	Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. (en)
title	wds:Q105109905-AAD10495-E0B1-4BE6-8AF2-24B204766431
title	Cloning, characterization, and expression in Escherichia coli of the Streptomyces clavuligerus gene encoding deacetoxycephalosporin C synthetase. (en)
page(s)	wds:Q105109905-F224DABB-9B3D-49CC-A620-D3410BAAB325
page(s)	754-760
instance of	wds:Q105109905-DBD3617B-73FE-4AA5-8F3D-C79C1A105D4C
instance of	scholarly article
main subject	wds:Q105109905-21557C8B-38EC-4983-A6C5-E2864DDB3CC7 wds:Q105109905-454008D3-7136-4EA1-ABAB-3AC6F1650AE6 wds:Q105109905-4BDF3CB8-F344-488C-B0ED-B1BF4CD2BDB5 wds:Q105109905-F2061EF8-3F8E-4082-BAAB-A0E7BD7665A0
main subject	Escherichia coli Streptomyces clavuligerus Cephalosporin (6S,7S)-3-(acetyloxymethyl)-7-[(5-amino-5-carboxypentanoyl)amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
published in	wds:Q105109905-EB2B1157-D20C-4EFE-9B0A-1C8A1F7C6B49
full work available at URL	wds:Q105109905-C4FF7CAF-B652-48B9-9A29-D6B4D53658D6
published in	Journal of Bacteriology
full work available at URL	https://europepmc.org/articles/pmc209661?pdf=render
issue	wds:Q105109905-C2F8C940-5CE6-44D3-AACB-DA04A3185CAF
volume	wds:Q105109905-A8CEFC3F-8349-4BF3-8B97-0A47A2C66735
issue	2
volume	171
number of pages	wds:Q105109905-06FD5021-FCA3-4C67-AF15-F6A6AFAA6E6A
number of pages	7 (xsd:decimal)
DOI	wds:Q105109905-F4E4C1AB-85CC-4FED-91A3-151C7188F5A1
DOI	http://dx.doi.org/10.1128/JB.171.2.754-760.1989
DOI	10.1128/JB.171.2.754-760.1989
online access status	wds:Q105109905-07EFD15E-4024-4ACE-B847-52F79AC0FB84
online access status	open access
is about of	https://www.wikidata.org/wiki/Special:EntityData/Q105109905
is cites work of	Reconstitution of TCA cycle with DAOCS to engineer Escherichia coli into an efficient whole cell catalyst of penicillin G. Kinetic analysis of cephalosporin biosynthesis in Streptomyces clavuligerus A dioxygenase gene (Ids2) expressed under iron deficiency conditions in the roots of Hordeum vulgare Modification of vertebrate and algal prolyl 4-hydroxylases and vertebrate lysyl hydroxylase by diethyl pyrocarbonate. Evidence for histidine residues in the catalytic site of 2-oxoglutarate-coupled dioxygenases Identification of arginine-700 as the residue that binds the C-5 carboxyl group of 2-oxoglutarate in human lysyl hydroxylase 1. The enzymes of β-lactam biosynthesis Structure of a cephalosporin synthase FeII/alpha-ketoglutarate-dependent hydroxylases and related enzymes The HAG mechanism: a molecular rationale for the therapeutic application of iron chelators in human diseases involving the 2-oxoacid utilizing dioxygenases

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