About: Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element

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description	article (en) ലേഖനം (ml) wetenschappelijk artikel (nl) наукова стаття, опублікована в червні 1997 (uk) article scientifique publié en 1997 (fr) im Juni 1997 veröffentlichter wissenschaftlicher Artikel (de)
publication date	wds:Q56267595-A94FBA42-319C-4A3E-A1E7-588BE07A485C
publication date	1997-06-01 00:00:00Z (xsd:dateTime)
language of work or name	wds:Q56267595-19C3643B-6FD5-40BC-906B-694E5AF3A0BD
language of work or name	English
cites work	wds:Q56267595-11E0506B-2F61-4138-B662-795985EB38FD wds:Q56267595-15909C2D-D5BD-4A9A-99B8-31B226C993A0 wds:Q56267595-2965D5C8-CCAE-468F-8EAF-F8AA1FD5A9FB wds:Q56267595-3AE7A45D-532E-4DB6-899F-06B088C2E96A wds:Q56267595-3F30F7DB-22E1-406C-A674-C3BA3A69A7AD wds:Q56267595-57039897-13F8-4BBB-8E75-D31633CDFA0D wds:Q56267595-583673FF-33B2-4BC2-B966-7ADE2204FDAF wds:Q56267595-5AA126E8-1BAF-4472-B863-2F9DC7273FE4 wds:Q56267595-6F92AD93-4484-4650-A949-7E99E9816970 wds:Q56267595-73DC9193-69F2-4863-8ACF-33439559091D wds:Q56267595-7725F9A8-D844-4AB3-90FB-AB27F79A06E1 wds:Q56267595-8312D177-4A47-4764-839F-284657248723 wds:Q56267595-8FF85556-1013-4C91-8512-B2535D3819BA wds:Q56267595-996A9B09-C975-4AAF-8F74-CD453938EDE0 wds:Q56267595-9BB8CDE7-6FE6-4068-859F-B24F7545AF68 wds:Q56267595-AE8D410F-F28B-4F60-BE36-1CADEE1933F0 wds:Q56267595-B155D9F6-1E0F-440E-9AC4-35067E9B14F7 wds:Q56267595-BCD631E1-2DD8-464E-AD19-55A0AF1C6DB1 wds:Q56267595-C875EEA6-2283-4475-B5B7-189309DA1359 wds:Q56267595-C87CD7E1-5E06-4418-9DFB-17735BF03CC4 wds:Q56267595-CA347A81-B384-4F5D-952E-5872324D418A wds:Q56267595-D1812209-4CC0-477E-89BC-DA2BA9B4BC86 wds:Q56267595-E1E7F70D-E23A-4260-BC8D-F98597FF4750 wds:Q56267595-EEB95EDD-EE1E-4901-ADA7-A38C8E6829F1
cites work	Use of T7 RNA polymerase to direct expression of cloned genes Protein splicing of the yeast TFP1 intervening protein sequence: a model for self-excision Single-step purification of bacterially expressed polypeptides containing an oligo-histidine domain. Intervening sequences in an Archaea DNA polymerase gene In vitro protein splicing of purified precursor and the identification of a branched intermediate Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin degradation Protein splicing converts the yeast TFP1 gene product to the 69-kD subunit of the vacuolar H(+)-adenosine triphosphatase Synthesis of proteins by native chemical ligation Molecular cloning of a brain-specific calcium/calmodulin-dependent protein kinase Primary structure and genetic organization of phage T4 DNA ligase Gene fusion expression systems in Escherichia coli Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein Cloning and characterization of the Bg/II restriction-modification system reveals a possible evolutionary footprint Protein splicing in the maturation of M. tuberculosis recA protein: a mechanism for tolerating a novel class of intervening sequence Protein splicing: evidence for an N-O acyl rearrangement as the initial step in the splicing process Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system A single step purification for recombinant proteins Characterization of a microtubule associated protein (MAP 2) fragment which associates with the type II cAMP‐dependent protein kinase Protein splicing: characterization of the aminosuccinimide residue at the carboxyl terminus of the excised intervening sequence The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli. Molecular characterization of a cyclosporin A-insensitive cyclophilin from the parasitic nematode Brugia malayi. Fusions to staphylococcal protein A Cloning the BamHI restriction modification system
author name string	wds:Q56267595-1050FEB5-274A-47AE-B05D-2FD4AEAD8FE5 wds:Q56267595-1FFB6F66-C2D2-47EE-9221-84BAF3D9CE5B wds:Q56267595-2905A456-D74E-4BFC-B030-56FBFA8B0C2C wds:Q56267595-36FC5EDA-18BF-4FAD-ACCB-C003BD340D7D wds:Q56267595-63AB19DB-4B09-458F-A4E5-4D6F817389EF wds:Q56267595-6B25986D-25AD-4916-AAAD-209BF49E07A7 wds:Q56267595-74853E6B-1E99-422F-9FDA-DF1B5FDB43DF wds:Q56267595-A0F4B465-6E9E-42B5-9BB4-66923D2F6D14 wds:Q56267595-AC9F9816-9A8A-4138-ADAD-26B932BA0F51 wds:Q56267595-BDE1B656-72EC-4A66-8B4E-E07B14CB343C wds:Q56267595-DA28451C-537D-44A7-98EE-77A84B5D4FB3 wds:Q56267595-E3DF3C1D-0EC8-4FF1-89C2-173283E74BFC wds:Q56267595-ECBA17D2-61AE-4C3F-80C3-C0181CBB79E6
author name string	S Chong M E Scott H Paulus M Q Xu D Landry J Benner R B Kucera J J Pelletier D G Comb F B Perler L M Vence C A Hirvonen F B Mersha
rdfs:label	Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (en) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (nl)
skos:prefLabel	Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (en) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (nl)
name	Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (en) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (nl)
title	wds:Q56267595-17F50D5F-7CE3-479D-BE2D-F54D54E98F6A
title	Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element (en)
page(s)	wds:Q56267595-D875376A-C406-4A59-ABA4-FFE6B152EEAF
page(s)	271-281
instance of	wds:Q56267595-75AB5810-CFA4-4CBC-A175-14AB49731CB1
instance of	scholarly article
PubMed ID	wds:Q56267595-D75636A1-3A8F-4E93-A746-F0DD7A90BAF1
PubMed ID	http://rdf.ncbi.nlm.nih.gov/pubchem/reference/9224900
PubMed ID	9224900

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